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Determination of cryptotanshinone,tanshinoneI and tanshinoneII_A of 9 kinds of Salviae Miltiorrhizae Radix et Rhizoma preparations by HPLC

Furong Qiu

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Abstract

AIM To estabilsh an HPLC method for determining cryptotanshinone,tanshinone ⅡA and tanshinoneⅠ in 9 kinds of Salviae Miltiorrhizae Radix et Rhizoma preparations.METHODS Separation was accomplished on the Agilent Eclipse XDB-C18 column(5 μm,4.6 mm× 150 mm),with 20 mmol/L ammonium acetate-acetonitrile(30∶70,v/v) as mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was set at 270 nm.RESULTS The three compounds showed a good linear relationship in the range of 0.19-3.00 μg/mL.The intra-day precision and inter-day precision RSDs of cryptotanshinone,tanshinoneⅠand tanshinoneⅡA were less than 15%,and the recoveries were between 85%-115%.CONCLUSION The method is accurate in the determination of cryptotanshinone,tanshinoneⅠ,tanshinoneⅡA in Salviae Miltiorrhizae Radix et Rhizoma preparations.

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AIM To estabilsh an HPLC method for determining cryptotanshinone,tanshinone ⅡA and tanshinoneⅠ in 9 kinds of Salviae Miltiorrhizae Radix et Rhizoma preparations.METHODS Separation was accomplished on the Agilent Eclipse XDB-C18 column(5 μm,4.6 mm× 150 mm),with 20 mmol/L ammonium acetate-acetonitrile(30∶70,v/v) as mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was set at 270 nm.RESULTS The three compounds showed a good linear relationship in the range of 0.19-3.00 μg/mL.The intra-day precision and inter-day precision RSDs of cryptotanshinone,tanshinoneⅠand tanshinoneⅡA were less than 15%,and the recoveries were between 85%-115%.CONCLUSION The method is accurate in the determination of cryptotanshinone,tanshinoneⅠ,tanshinoneⅡA in Salviae Miltiorrhizae Radix et Rhizoma preparations.

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Available abstract

AIM To estabilsh an HPLC method for determining cryptotanshinone,tanshinone ⅡA and tanshinoneⅠ in 9 kinds of Salviae Miltiorrhizae Radix et Rhizoma preparations.METHODS Separation was accomplished on the Agilent Eclipse XDB-C18 column(5 μm,4.6 mm× 150 mm),with 20 mmol/L ammonium acetate-acetonitrile(30∶70,v/v) as mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was set at 270 nm.RESULTS The three compounds showed a good linear relationship in the range of 0.19-3.00 μg/mL.The intra-day precision and inter-day precision RSDs of cryptotanshinone,tanshinoneⅠand tanshinoneⅡA were less than 15%,and the recoveries were between 85%-115%.CONCLUSION The method is accurate in the determination of cryptotanshinone,tanshinoneⅠ,tanshinoneⅡA in Salviae Miltiorrhizae Radix et Rhizoma preparations.

Key concepts: Radix (gastropod), Chemistry, Chromatography, High-performance liquid chromatography, Ammonium acetate, Biology, Botany

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Determination of cryptotanshinone,tanshinoneI and tanshinoneII_A of 9 kinds of Salviae Miltiorrhizae Radix et Rhizoma preparations by HPLC — Research Paper | ScholarLens