Effect of Eye acupuncture therapy on BDNF expression in rats with hippocampus ischemia-reperfusion injury at different time periods

Objective: To investigate time-effect relationship between eye acupuncture and curative effect of CIRI and the possible mechanisms.Methods: Establishing the rat model of CIRI with suture method.3h,24h and 72h after reperfusion,the neurophysical behaviours were accessed by ZeaLonga neurophysical impairment marks.The methods of Western-blotting and RQ-PCR were taken to detect the alteration of the BDNF protein and BDNF mRNA in rat hippocampus after the eye acupuncture therapy.Results: The expression of the BDNF protein and BDNF mRNA in rat hippocampus after the eye acupuncture therapy was strongly increased at 3h,24h and 72h.The expression of the BDNF at 24h were higher than those at other periods(P0.01).Conclusion: The eye acupuncture therapy can improve the CIRI evidently,the curative effect at 24h is superior to that at 3h and at 72h.It is related to increasing the expression of hippocampus BDNF..