Inhibitive effect of resveratrol on proliferation of human gastric cancer MGC-803 cells

Objective To explore the effect and mechanism of resveratrol on cell cycle of human gastric cancer MGC-803 cells.Methods The blank control group,low dose resverstrol group(25 μg/mL) and high dose resverstrol group(50 μg/mL) were arranged in the experiment.After the administering of resverstrol to experimental groups for 24 hr,the inhibition rate of cell proliferation was measured with methyl-thiazolyl-tetrazolium method.The colony forming and the cell cycle were analyzed with flow cytometry,and the mRNA expression of bcl-2,c-myc gene were determined with RT-PCR method.Results Resveratrol could markedly inhibit proliferation of human gastric cancer cells compared with that of the blank control group.The cell cycle was blocked to G0/G1.The mRNA level of bcl-2 and c-myc gene were decreased compared with that of the blank control group.Conclusion Resverstrol could inhibit cell proliferation and block the cell cycle to G0/G1 in human gastric cancer MGC-803 cells.The effects might be related to decreased expression of bcl-2 and c-myc gene.