Cloning and Sequence Analysis of F3H Gene cDNA from Longan Embryo

The protein of 'Lidongben' longan zygotic embryos during different development stages were separated and compared by IEF/SDS-PAGE technique.Flavanone 3-hydroxylase was one of differential proteins identified by MALDI-TOF/TOF.Then F3H gene cDNA sequence was cloned from longan zygotic embryos using RT-PCR and RACE technique.A 1 404 bp full-length cDNA sequence was obtained.Analysis of F3H gene cDNA indicated that it encoded a peptide containing 365 amino acids.The nucleotide sequence comparison with the F3H gene of Citrus sinensis,Gossypium hirsutum and Pyrus communi showed that identity was all higher than 80%.The sequence was accepted and released by GenBank(Accession number:EF468104).