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2009•Journal of Fujian Agriculture and Forestry UniversityRequires access

Cloning and sequence analysis of the full-length cDNA of glyceral-dehyde-3-phosphate dehydrogenase gene from longan cotyledon embryo

Lu BingGuo, He WeiYi, Yanhong Shen, Jiang JiMou, Xiaojing Chen, Wei Chen, LüLiuXin

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Abstract

On the basis of one cDNA-AFLP expressed sequence tag(EST) from longan(Dimocarpus longan Lour.) cotyledon embryos which is 84%homologous to Antirrhinum majus,the full length cDNA of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene was cloned by rapid-amplification of cDNA ends(RACE).The gene consisted of 1395 bp encoding a protein of 336 amino acids,with 71 bp and 316 bp at 5′-UTR and 3′-UTR,respectively.The amino acid sequence comparison with the GAPDH gene of Oryza sativa,Vitis vinifera,Musa acuminata,Arabidopsis thaliana,and Ginkgo biloba showed that identity was all higher than 85%.The sequence was accepted and released by GenBank(accession number: FJ694011).

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What this paper is about

On the basis of one cDNA-AFLP expressed sequence tag(EST) from longan(Dimocarpus longan Lour.) cotyledon embryos which is 84%homologous to Antirrhinum majus,the full length cDNA of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene was cloned by rapid-amplification of cDNA ends(RACE).The gene consisted of 1395 bp encoding a protein of 336 amino acids,with 71 bp and 316 bp at 5′-UTR and 3′-UTR,respectively.The amino acid sequence comparison with the GAPDH gene of Oryza sativa,Vitis vinifera,Musa acuminata,Arabidopsis thaliana,and Ginkgo biloba showed that identity was all higher than 85%.The sequence was accepted and released by GenBank(accession number: FJ694011).

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Available abstract

On the basis of one cDNA-AFLP expressed sequence tag(EST) from longan(Dimocarpus longan Lour.) cotyledon embryos which is 84%homologous to Antirrhinum majus,the full length cDNA of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene was cloned by rapid-amplification of cDNA ends(RACE).The gene consisted of 1395 bp encoding a protein of 336 amino acids,with 71 bp and 316 bp at 5′-UTR and 3′-UTR,respectively.The amino acid sequence comparison with the GAPDH gene of Oryza sativa,Vitis vinifera,Musa acuminata,Arabidopsis thaliana,and Ginkgo biloba showed that identity was all higher than 85%.The sequence was accepted and released by GenBank(accession number: FJ694011).

Key concepts: Complementary DNA, Biology, Molecular biology, Gene, Rapid amplification of cDNA ends, Accession number (library science), GenBank, Nucleic acid sequence

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Cloning and sequence analysis of the full-length cDNA of glyceral-dehyde-3-phosphate dehydrogenase gene from longan cotyledon embryo — Research Paper | ScholarLens