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Study on Extraction and Purification of Polysaccharides from Cordyceps militaris

Ping Zhang

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Abstract

The medium mainly containing rice and the pupa of silkworm was used to cultivate the sporocarp of Cordyceps militaris.Extraction processing of water-soluble polysaccharide in Cordyceps militaris was studied through orthogonal experiment,and the anthrone-sulphuric acid method was used to determine polysaccharide content.Three times(3 hours per times) extraction with 20 times water at 90 ℃ was the best extraction conditions.Simultaneously deproteinization effect was examined with different methods.

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What this paper is about

The medium mainly containing rice and the pupa of silkworm was used to cultivate the sporocarp of Cordyceps militaris.Extraction processing of water-soluble polysaccharide in Cordyceps militaris was studied through orthogonal experiment,and the anthrone-sulphuric acid method was used to determine polysaccharide content.Three times(3 hours per times) extraction with 20 times water at 90 ℃ was the best extraction conditions.Simultaneously deproteinization effect was examined with different methods.

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Available abstract

The medium mainly containing rice and the pupa of silkworm was used to cultivate the sporocarp of Cordyceps militaris.Extraction processing of water-soluble polysaccharide in Cordyceps militaris was studied through orthogonal experiment,and the anthrone-sulphuric acid method was used to determine polysaccharide content.Three times(3 hours per times) extraction with 20 times water at 90 ℃ was the best extraction conditions.Simultaneously deproteinization effect was examined with different methods.

Key concepts: Cordyceps militaris, Polysaccharide, Extraction (chemistry), Anthrone, Cordyceps, Pupa, Water extraction, Chemistry

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