Extraction processing optimization of polysaccharides from Cordyceps militaris L.Link by compound enzyme

This research aimed to optimize the extraction process of polysaccharides from Cordyceps militaris L.Link by the compound enzyme made from papaya protease and cellulase in certain proportion.Using the orthogonal experiment with single factor experiment as the foundation,the influencing factors of polysaccharide extracted from Cordyceps militaris L.Link was discussed as follow: extraction temperature,extraction time and ratio of solid to liquid.The optimum extraction process of polysaccharides from Cordyceps militaris L.Link was as follow: water extracted temperature 80 ℃,time 2 h,ratio of solid to liquid 1:25.The result can offer a theoretical basis for functional food development of Cordyceps militaris L.Link.