Effects of Ulinastation on Serum TNF-α, IL-6 in Rats after Intestinal Ischemia-reperfusion

Objective: To evaluate the protective effect and mechanism of ulinastatin on intestinal ischemia-reperfusion injury by detecting the changes of TNF-α IL-6 in serum levels. Methods: Eighty-four healthy Wistar rats were randomly divided into sham operation group(C), intestinal ischemia-reperfusion group(I), and the UTI treatment groups(U). Intestinal ischemia model was produced by occlusion of the superior mesenteric artery(SMA) for 60 min. Then Group I and Group U divided into 0min, 2 h and 6 h group respectively according to the ischemia reperfusion time. In group I and group U, the rats were treated with normal saline(2 mL) UTI(5×104U/kg) via the tail vein. In sham-operated group, SMA was separated, but without occlusion. The serum concentration of TNF-α IL-6in the abdominal aorta was detected at above time point respectively. Results: Intestinal ischemia-reperfusion at each time point resulted in serum TNF-α, IL-6 changes. Compared with that in the sham group, the serum concentration of TNF-α and IL-6 in group I and group U increased obviously at all time points(P0.01). Compared with the corresponding time point of group I, the serum concentration of TNF-α at 0 min and 2 h in group U decreased significantly(P0.01), and the serum concentration of IL-6 at 0 min, 2 h and 6h in group U decreased(P0.05). Conclusion: Ulinastatin can reduce the inflammatory response in the small intestine ischemia reperfusion.