Effects of pirinixic acid on serum TNF-α and IL-1β in rats with hepatic ischemia-reperfusion injury

Objective To observe the effects of pirinixic acid(Wy14643) on serum TNF-α and IL-1β in rats with hepatic ischemia-reperfusion(I-R) injury.Methods Thirty male Sprague-Dawley rats weighing 220-280 g were equally randomized into five groups: Wy14643 1 mg/kg + I-R group(group W1),Wy14643 5 mg/kg + I-R group(group W2),Wy14643 10 mg/kg +I-R group(group W3),sham operation group(group S) and I-R group(group I-R).Hepatic I-R injury model was established by clamping blood supply of the left and median lobes of the liver with vascular clamps for 90 min.Rats in group S were done the same operation without clamping blood vessels.Rats in groups W1,W2 and W3 were intraperitoneally administered with the dose of Wy14643 1,5 and 10 mg/kg 60 min before ischemia,respectively.In groups S and I-R,rats were given the same volume of saline at the same time.Blood samples were taken from abdominal aorta 4 h after reperfusion to measure the serum levels of TNF-α and IL-1β.Results Four hours after hepatic I-R,group S had lower serum TNF-α and IL-1β compared with groups W1,W2,W3 and I-R(P0.05).Groups W2 and W3 had lower serum TNF-αand IL-1βcompared with group I-R(P0.05).Conclusion Pretreatment with pirinixic acid can reduce the serum levels of TNF-α and IL-1β in rats with hepatic ischemia-reperfusion injury.