Optimization of Enzymatic Hydrolysis of Defatted Silkworm Pupa by Animal Proteases

Response surface methodology (RSM) was applied to optimize enzymatic hydrolysis of defatted silkworm pupa protein by commercial animal proteases. Effects of hydrolysis temperature, pH, protease amount and material-liquid ratio on the degree of hydrolysis (DH) were evaluated by single factor experiments. Results indicated that a linear relationship between hydrolysis temperature or protease amount and the degree of hydrolysis, a square effect of temperature, pH or material-liquid ratio on the degree of hydrolysis, cross-interaction of temperature and pH on the degree of hydrolysis were observed. The optimal hydrolysis condition for silkworm pupa protein was 45 ℃ of hydrolysis temperature, pH 7.56, 4.24% protease amount, and 1:29.7 of material-liquid ratio for 8 h of hydrolysis. The degree of hydrolysis of silkworm pupa protein was 48.24% under this optimal condition.