Optimization of protease hydrolysis conditions on silkworm pupa protein

Purpose: Enzyme hydrolysis of silkworm pupa protein was studied in order to provide references to deeply exploit silkworm pupa protein.Methods: Protease was used to hydrolyze silkworm pupa protein and spectrophotometry was used to detect indicators.Results: When hydrolyzed by single protease,dedicated protease for silkworm pupa protein showed the highest hydrolysis degree out of 8 proteases used in the experiment,the best hydrolysis conditions were: the substrate concentration was 7%,53℃,pH7.2,the additive amount of protease was 3%,and the hydrolysis degree turned out to be 21.44%.