CONSTRUCTION OF DIFFERENTIALLY EXPRESSED cDNA LIBRARIES FROM DISSENMILATORY MALIGNANT TRANSFORMED HUMAN BRONCHIAL EPITHELIAL CELLS INDUCED BY ALPHA-PARTICLE RADIATION

Purpose: To construct differentially expressed cDNA libraries from different malignant transformed human bronchial epithelial cells induced by alpha-particle radiation. Methods: Suppression subtractive hybridization (SSH). Results: Three differentially expressed cDNA libraries were constructed from different malignant transformed human bronchial epithelial cells. The number of clones is 416 in A subtraction library (The cDNAs of BEP2D cells as tester and R15Hp35 cells as driver), 301 in B subtraction library (The cDNAs of R15Hp20 cells as tester and R15Hp35 and BEP2D cells mixed together as driver.) and 568 in C subtraction library (The cDNAs of R15Hp35 cells as tester and BEP2D cells as driver). After 70 cDNAs were sequenced and analyzed, 61 cDNAs were found to be known genes, and 9 cDNAs were found to be novel ones. Conclusion: The cDNAs of three subtraction libraries may represent differentially expressed cDNA of different malignant transformed human bronchial epithelial cells induced by alpha-particle radiation. The data provide a basis for further investigation of the molecular mechanism of lung cancer induced by alpha-particle radiation.