Common pathogens isolated from blood culture specimens and their distribution and drug resistance

Objective To investigate the distribution and the drug resistance of pathogens in blood culture inour hospital over the past 5 years.Methods The blood samples were cultivated by using the Bactec 9050 system.Isolated colonies were identified and drug sensitivity was tested by ATB Expression of Bio-Merieux of France,and the extended spectrum β-lactamases of E.coli and K.pneumoniae were detected by the method recommended in CLSI.Results Gram-positive and Gram-negative bacteria accounted for 48.9% and 43.8%,respectively,and fungi for 7.3% in 252 strains isolated from blood specimens.The most frequent Gram-negative bacteria isolated were E.coli of 66 strains,and accounting for 26.2%,Salmonella of 46 strains,and accounting for 18.3%,K.pneumoniae of 34 strains,and accounting for 13.5%.ESBLs-producing E.coli strains and K.pneumoniae accounted for 24.2% and 20.6%,respectively.The most frequent gram-positive pathogenic bacteria isolated were Staphylococcus aureus of 31 strains,and accounting for 12.3% and coagulase negative Staphylococcus coagulase of 25 strains,and accounting for 9.9%.The MRSA occurrence rate was 9.7 % and the occurrence of MRCNS was 80.0%.No Gram-positive cocci strains were resistant to vancomycin.Conclusion The pathogens distribution of blood culture are variable in different area.Gram-negative bacilli is the leading pathogens in our hospital and the infection rate of Salmonella is high,which is sig-nificant for the epidemiology.It can instruct doctors to utilize antibiotics more reasonably and also guide to formulateexperiential therapeutic regimen.