Study on conversion of banana stalk into sugar by Trichoderma reesei and Apsergillus niger

The characters of cellulase production by Trichoderma reesei and Apsergillus niger using banana stalk as the substrate was studied.The activity complementary emzye system was obtained when Trichoderma reesei was cultivated 30 h below 30 ℃,then mixed culture of Trichoderma reesei and Apsergillus niger at 32 ℃.The activity of FPA and β-Gluase reached 920.6 U/g and 864.2 U/g,respectively.The result of saccharification of banana stalk by inulinase showed: the highest conversion coefficient of 30.6% was obtained when the saccharification was carried out at the ratio of two strains inulinase was 2∶1,and conversion coefficient was enhanced 16.8% than pure culture of Trichoderma reesei;The conversion coefficient by mixed culture was 31.5%,4 h was shortened and conversion coefficient was enhanced 20.2% under the optimal conditions.It indicated that mixed culture not only optimize the composition of cellulase,enhance the saccharification efficiency,but also simplify the production process of the cellulose greatly.