2009•Linchan huaxue yu gongyeRequires access
Cellulase Production from Mixed Fermentation of Trichoderma reesei RUT C-30 and Apsergillus niger NL02
Hao Fang, Xiangyang Song, Chen Zhao, Chang Zheng, Jie Chu, Yong Qiang
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Abstract
Cellulase production from mixed fermentation of Trichoderma reesei and Aspergillus niger was investigated.The balance point of the two strains was established by delaying inoculation time of A.niger and using different inoculum ratios.The time courses of cellulase production by monoculture of T.reesei and A.niger were used as controls.It was found that the highest filter paper activity(FPA),3.295 IU/mL,was obtained when delay in A.niger inoculation was 48h and volumetric inoculum ratio of T.reesei versus A.niger was 5∶1,higher than the FPA of the monoculture of T.reesei,2.480IU/mL.The β-glucosidase activity(β-GA) also increased from 0.243IU/mL of the monoculture of T.reesei to 1.010IU/mL of the mixed culture aforementioned.It was demonstrated that the mixed fermentation of T.reesei and A.niger was feasible and superior to the monoculture of T.reesei or A.niger.