EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY OF COMPOUND CHLORHEXIDINE ACETATE DISINFECTANT
Yang Hong-cai
Abstract
Yang Hong-cai
Abstract
In order to know the germicidal efficacy of compound chlorhexidine acetate disinfectant,suspension quantitative germicidal test and field trial were used to carry out experimental observation.Results: The killing logarithm values of Staphylococcus aureus and Escherichia coli exposed to the stock solution of the compound disinfectant containing chlorhexidine acetate 1540 mg/L for 3 min were 5.0.Spraying moistening disinfection of hands and forearms with the disinfectant for 2~3 min could kill the natural bacteria by a logarithm 1.0 in average.Aerosol spraying disinfection of a 45 m~3 room with the stock solution of the disinfectant at a dosage of 8 ml/m~3 for 30 min caused 97.5% eradicating rate of natural bacteria in air.After the stock solution of the disinfectant was stored in sealed contition at 37℃ for 90 days,its chlorhexidine acetate content decreased by 1.94%.Conclusion: The disinfectant is stable and has good efficacy in killing vegetative forms of bacteria and natural bacteria on skin.
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In order to know the germicidal efficacy of compound chlorhexidine acetate disinfectant,suspension quantitative germicidal test and field trial were used to carry out experimental observation.Results: The killing logarithm values of Staphylococcus aureus and Escherichia coli exposed to the stock solution of the compound disinfectant containing chlorhexidine acetate 1540 mg/L for 3 min were 5.0.Spraying moistening disinfection of hands and forearms with the disinfectant for 2~3 min could kill the natural bacteria by a logarithm 1.0 in average.Aerosol spraying disinfection of a 45 m~3 room with the stock solution of the disinfectant at a dosage of 8 ml/m~3 for 30 min caused 97.5% eradicating rate of natural bacteria in air.After the stock solution of the disinfectant was stored in sealed contition at 37℃ for 90 days,its chlorhexidine acetate content decreased by 1.94%.Conclusion: The disinfectant is stable and has good efficacy in killing vegetative forms of bacteria and natural bacteria on skin.
Key concepts: Disinfectant, Stock solution, Chlorhexidine, Bacteria, Microbiology, Chemistry, Staphylococcus aureus, Chromatography