Extraction and purification of polyphenols in Ilex paraguarensis and its antibacteriostatic activities
Xiong Jian-hua
Abstract
Xiong Jian-hua
Abstract
The refluence extraction and purification procedure of polyphenols in Ilex paraguarensis were investigated with the content of polyphenols as index;the antibacteriostatic activities against pathogenic bacteria in diet were estimated using K-B filter paper slip method. The results showed that the optimum extraction conditions were 10 fold of 70% ethanol,dip in 80 ℃ water,refluxing 60 min;EtoAc was the best reagent for purification, the content of polyphenols was 80.64% after purification;the pure polyphenols showed considerable antibacterial effect.
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The refluence extraction and purification procedure of polyphenols in Ilex paraguarensis were investigated with the content of polyphenols as index;the antibacteriostatic activities against pathogenic bacteria in diet were estimated using K-B filter paper slip method. The results showed that the optimum extraction conditions were 10 fold of 70% ethanol,dip in 80 ℃ water,refluxing 60 min;EtoAc was the best reagent for purification, the content of polyphenols was 80.64% after purification;the pure polyphenols showed considerable antibacterial effect.
Key concepts: Polyphenol, Extraction (chemistry), Chemistry, Chromatography, Reagent, Ethanol, Food science, Biochemistry