Korean Pine Species of Scale Secondary Purification Process of Polyphenols and Oxidation Resistance

[Objective]The research aimed to test and verify secondary purification and antioxidant properties of Korean pine scale polyphenols. [Method]By polyamide column chromatography method for AB-8 resin purification of Korean pine scale polyphenols,liquid after secondary purification was purified,and DPPH free radical clearance rate and the total reducing power were determined. [Result]Polyamide was obtained by the orthogonal experiment analysis. The optimal conditions for secondary purification was as follows: 30 to 60 mesh polyamide resin,sample pH 5,adsorption time1 h,eluent for ethanol 2 BV on sample amount,elution concentration 70%,elution pH 7. In this process,the purity of polyphenols was up to 62% ± 2%. IC50 value of secondary purified polyphenols removal DPPH free radical was 10. 94 μg / ml,total reducing power was similar to VCand slightly higher than the fluid of a purified polyphenols. [Conclusion] Korean pine species of scale secondary purified polyphenols purity was about 2 times a purified polyphenols,and had antioxidant properties.