Extraction and Purification of Polyphenols from Ilex paraguarensis and Its Effects on Activity of Ptyalin

To detemine the optimum extraction and purification process of polyphenols from Ilex paraguarensis,then to estimate the pure polyphenols' effects on activity of ptyalin,the refluence extraction and purification process of polyphenols from Ilex paraguarensis were investigated with the content of polyphenols as index;effects of the different content pure polyphenols on activity of ptyalin were compared using idodine-starch spectrophotometrical method.The results showed that the optimum extraction conditions were 30 fold of 70% ethanol,dip in 80 ℃ water,refluxing 60 min;EtoAc was the best reagent for purification,the content of polyphenols was 80.04% after purification;The pure polyphenols could restrain activity of ptyalin.