Mechanism of Histone Deacetylase Inhibitor Trichostatin A Induced Apoptosis in Human Osteosarcoma Cells MG-63

Objective To explore the apoptosis mechanism of MG-63 cell induced by TSA.Methods The MTT method was used to test MG-63 cell growth.The cell cycle were test by FCM.Immunochemistry were used to observe the expression of survivin gene and VEGF in MG-63 cell line after treated by TSA.Semi-quantitative RT-PCR was used to detect the mRNA expressions of P21waf1/cip1.Results MG-63 cell were very sensitive to TSA which inhibited the growth of cell in a concentrantion-dependent manner.Flow cytometry data indicated that the increases in the G2/M phase cell percentage and concomitant fall in the percentage of G0/G1 phase cells.By increasing the TSA concentration,the expression of survivin and VEGF decreased gradually.After be treated 24h by TSA,the express of P21waf1/cip1mRNA was increased in MG-63 cell.Conclusion TSA effect the activity of cyclin-dependent kinase through increase the express of P21waf1/cip1mRNA.TSA make MG-63 cells blockage G2/M phase and inhibit cell proliferation of MG-63 cells.