2012•Journal of Analytical ScienceRequires access

Spectrophotometric Method for the Determination of Chloramphenicol by Alizarin Red

Hong Tao Jiang

Abstract

The complex reaction of chloramphenicol(CHP) and alizarin red(ALZR) was investigated by spectrophotometry.The optimum experimental conditions were established,and a simple and rapid method for the determination of chloramphenicol has been developed.Results showed that the ratio of chloramphenicol and alizarin red in the composition of complex was 1∶1 in Britton-Robinson(B-R)-ethanol solution at room temperature.The apparent molar absorptivity of the complex was 1.19×104 L/(mol·cm)at 474 nm.The Beer's law was obeyed in the range of 2.0×10-7~3.0×10-5 mol/L for chloramphenicol with a correlation coefficient of 0.9985.The relative standard deviation was 0.86%(n=10),and the recovery was 99.4%~101.2%.

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Key concepts: Chloramphenicol, Chemistry, ALIZARIN RED, Molar absorptivity, Spectrophotometry, Alizarin, Chromatography, Relative standard deviation