Color Reaction of Alizarin Blue S with Chloramphenicol and Its Applications

The color reaction of alizarin blue S with chloramphenicol was studied.A novel sensitive visible light spectrophotometric method for determination of chloramphenicol has been established.In the acidity Britton-Robinson buffer medium,alizarin blue S reacted with chloramphenicol to form ion-association complexes with increased absorption intensity(|ΔA|).The maximum plus absorption wavelength located at 638nm,and the apparent molar absorption coefficient(e) was 1.32×104L·mol-1·cm-1;The maximum negative absorption wavelength located at 566 nm,and the apparent molar absorption coefficient(e) was 8.00×103L·mol-1·cm-1;Beer's law was obeyed in the range of 0—2.0×10-5 mol·L-1.The optimum reaction conditions,the nature of analytical chemistry and the methodological precision and reliability were studied.The method has been applied to the determination of chloramphenicol in the city sell chloramphenicol medicament with satisfactory results.