2008•Jiguang zazhiRequires access

Simultaneous determination of purerarin and tanshinone in Tongmai granules by HPLC

Tian Guang-zhen

Abstract

Objective: To establish the method of controlling the quality of Tongmai granules.Method:High performance liquid chromatography(HPLC) was used.Column: Zorbax RP-C18 Chromatography column(150×4.6 mm,5μm);mobile phase: methanol-water(75∶25);flow speed: 0.5 mL/min;λ=254nm.Column temperature 25℃.Result: There was good linear relationship at the ranges of 22.5 μg/mL-225μg/mL,r=0.9997 and 16.5 μg/mL-165μg/mL,r=0.9998 for puerarin and tanshinone,respectively.Conclusion:The method is simple,stable and accurate,so it could be used to quality standards for Tongmai Granules.

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Key concepts: Chromatography, High-performance liquid chromatography, Puerarin, Chemistry, Methanol, Medicine, Alternative medicine, Pathology