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2009•Anhui Medical and Pharmaceutical JournalRequires access

Determination of harpagosid in qingfeiyin by HPLC

Weidong Chen

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Abstract

Aim A method was established for the determination of harpagosid in qingfeiyin by HPLC.Method The separation was performed on SinochromODS Cl8 column,The mobile phase was acetonitrile-water solution in linear gradient elution.The detection wavelength was 238 nm.The flow rate was 1.0 ml·min-1.Result The linear range of harpagosid was from 12.37~61.85 mg·L-1(r=0.999 9).The average recovery was 97.86%,and RSD was 1.42 %(n=5).Conclusion The method is simple and accurate.It can be used for the quality control of qingfeiyin.

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What this paper is about

Aim A method was established for the determination of harpagosid in qingfeiyin by HPLC.Method The separation was performed on SinochromODS Cl8 column,The mobile phase was acetonitrile-water solution in linear gradient elution.The detection wavelength was 238 nm.The flow rate was 1.0 ml·min-1.Result The linear range of harpagosid was from 12.37~61.85 mg·L-1(r=0.999 9).The average recovery was 97.86%,and RSD was 1.42 %(n=5).Conclusion The method is simple and accurate.It can be used for the quality control of qingfeiyin.

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Available abstract

Aim A method was established for the determination of harpagosid in qingfeiyin by HPLC.Method The separation was performed on SinochromODS Cl8 column,The mobile phase was acetonitrile-water solution in linear gradient elution.The detection wavelength was 238 nm.The flow rate was 1.0 ml·min-1.Result The linear range of harpagosid was from 12.37~61.85 mg·L-1(r=0.999 9).The average recovery was 97.86%,and RSD was 1.42 %(n=5).Conclusion The method is simple and accurate.It can be used for the quality control of qingfeiyin.

Key concepts: Chromatography, Gradient elution, Linear range, High-performance liquid chromatography, Acetonitrile, Recovery rate, Chemistry, Linear relationship

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