The Effect of touxietongluo on MMP-1 and TIMP-1 of hepatic tissue of hepatic fibrosis rats

Objective:To study the mechanism and effect of Touxietongluofa on hepatic fibrosis under the guidance of Zhukejiao theory.Methods:Male SD rats except the rats in the normal group,others were inflicted with liver fibrosis by intraperitoneal injection of 40% carbon tetrachloride,and were given medication at the same time.Rats were given normal saline in the normal group and the model group,colchicine in the positive group,Jiajiansanjiasan extractum in the Touxietongluo group.After eight weeks,all the rats were executed,and the hepatic tissues were kept for pathologic examination to observe hepatic fibrosis and immunohistochemical to detect MMP-1 and TIMP-1 of hepatic tissue.The experimental results were processed by image system and statistical software.Results:Compared with the model group,MMP-1 expression increased obviously and TIMP-1 expression decreased obviously in the hepatic tissues of rats in the Touxietongluo group(P0.01),while MMP-1 expression increased(P0.05) and TIMP-1 expression decreased obviously(P0.01) in rat liver of the positive group.Compared with the positive group,MMP-1 expression increased and TIMP-1 expression decreased in the hepatic tissues of rats in the Touxietongluo group(P0.05).Conclusions:Touxietongluo can obviously relieve the pathological damage in hepatic tissues of hepatofibrosis rats,and thus has a good curative effect of anti-hepatic fibrosis.Its therapeutic mechanism mainly maybe adjust the metabolism of extra-cellular matrix by increasing MMP-1 expression and decreasing TIMP-1 expression.