Development and application of monoclonal antibodies specific to hemagglutinin of subtype H5 and H9 avian influenza virus

A panel of monoclonal antibodies specific to hemagglutinin of subtype H5 and H9 avian influenza virus (AIV_H5 and AIV_H9) were developed by fusing between SP2/0 myeloma cells and spleen cells of Babl/c mice immunized with AIV_H5 and AIV_H9.Three of them were designated as 4B6,4A3,and 3H1 respectively,which were specific to AIV_H5,but not reacted with AIV_H9,Newcasttlet Disease virus from chickens(NDV),NDV from geese,adenovirus from geese and EDS 76 by hemagglutination inhibition test (HI) and immunofluorensense assay.Others were named as 6E6 and 5B4 respectively,which were against AIV_H9 but not reacted with other tested viruses.Chicken embryo fibroblast(CEF) cells infected with 10 TCID 50 of AIV_5 could been detected in 24 hours by these monoclonal antibodies in indirect immunofluorescent assay (IFA).The results suggested that these monoclonal antibodies will be very useful in control of avian influenza.