ScholarLens Back to search
2003•Journal of Yangzhou UniversityRequires access

DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC TO HEAMAGGLUTININ OF SUBTYPE H9 AVIAN INFLUENZA VIRUS

Qin Ai

Open publisher page 0 citations

Abstract

Three monoclonal antibodies (Mabs) specific to heamagglutinin of subtype H9 avian influenza virus (AIV-H9) were developed by cell fusing with the Balb/C mice immunized with AIV-H9. which were named as 6E6, 6B6, and 5B4, respectively. By heamagglutination inhibition (HI), the three Mabs specifically reacted with only AIV-H9 virus but not with subtype H5 avian influenza virus (AIV-H5), Newcastle disease virus (NDV), adenovirus and so on. Chicken embryo fibroblast (CEF) cells infected with AIV-H9 could be detected by the Mabs in immunofluorescence assay (IFA). The sensitivity of the virus detection by IFA was better than that of the virus isolation with chicken embryos. It indicated that these Mabs would be very useful in diagnosis and control of avian influenza.

About this research paper

What this paper is about

Three monoclonal antibodies (Mabs) specific to heamagglutinin of subtype H9 avian influenza virus (AIV-H9) were developed by cell fusing with the Balb/C mice immunized with AIV-H9. which were named as 6E6, 6B6, and 5B4, respectively. By heamagglutination inhibition (HI), the three Mabs specifically reacted with only AIV-H9 virus but not with subtype H5 avian influenza virus (AIV-H5), Newcastle disease virus (NDV), adenovirus and so on. Chicken embryo fibroblast (CEF) cells infected with AIV-H9 could be detected by the Mabs in immunofluorescence assay (IFA). The sensitivity of the virus detection by IFA was better than that of the virus isolation with chicken embryos. It indicated that these Mabs would be very useful in diagnosis and control of avian influenza.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Three monoclonal antibodies (Mabs) specific to heamagglutinin of subtype H9 avian influenza virus (AIV-H9) were developed by cell fusing with the Balb/C mice immunized with AIV-H9. which were named as 6E6, 6B6, and 5B4, respectively. By heamagglutination inhibition (HI), the three Mabs specifically reacted with only AIV-H9 virus but not with subtype H5 avian influenza virus (AIV-H5), Newcastle disease virus (NDV), adenovirus and so on. Chicken embryo fibroblast (CEF) cells infected with AIV-H9 could be detected by the Mabs in immunofluorescence assay (IFA). The sensitivity of the virus detection by IFA was better than that of the virus isolation with chicken embryos. It indicated that these Mabs would be very useful in diagnosis and control of avian influenza.

Key concepts: Virology, Monoclonal antibody, Virus, Influenza A virus subtype H5N1, Avian influenza virus, Biology, Newcastle disease, Antibody

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC TO HEAMAGGLUTININ OF SUBTYPE H9 AVIAN INFLUENZA VIRUS — Research Paper | ScholarLens