Expression of Tissue Factor Pathway Inhibitor(TFPI)mRNA in activated platelet in coronary heart disease(CHD) and the anticoagulant mechanism of low molecular weigh heparin(LMWH)for them

Objective To investigate the expression of TFPI mRNA in activated platelet in CHD and the anticoagulant mechanism of LMWH for them.Methods By means of RT - PCR method and ELASA technique,the activated pletelet mRNA levels and the levels of free - TFPI were measured in patient with stable angina(SA) group,unstable angina(UA), acute myocardial infarction(AMI) and control group in the study.Results(1 )The mRNA expression level of TFPI in platelet were signifeicant higher in patient with AMI and UA than that of healthy survey(P 0.05).(2) Contrasting to the control group,the level of free - TFPI in patient with AMI or UA were significantly higher(P 0.01).The average level of FTFPI of those three groups were 93.39,74.91 and 62.98μg/L.(3)There were positive correlation between the mRNA expression of TFPI in platelet and the change of plasma free -TFPI in patients with AMI,UA and SA.(4) After the treatment by LMWH, free - TFPI increased significantly among the AMI,UA and SA group.The average level of FTFPI of those three groups exposured to LMWH were 93.39、74.91 and 62.98μg/L.Conclusion The expression of TFPI were enhanced in CHD patient owing to platelet activation.TFPI were released from the pool of platelet into circulation when exposure to LMWH.