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Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase(PAL) Gene from Fagopyrum esculentum

Qi Wu

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Abstract

The cDNA ORF sequence of phenylalanine ammonia-lyase gene(FePAL) from Fagopyrum esculentum was cloned by RT-PCR.The cDNA sequence was 2169 bp in length,encoding 722 amino acids and had a high homology(80%-97%) with PAL genes from other plants.The deduced amino acid sequence of FePAL contained the typical sequence(GTTTASGDLVPLSYIA) in PAL activity center region,deamination residues and catalytic active sites.Phylogenetic tree analysis revealed that FePAL was most closely related to the PAL of Fagopyrum tataricum.

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What this paper is about

The cDNA ORF sequence of phenylalanine ammonia-lyase gene(FePAL) from Fagopyrum esculentum was cloned by RT-PCR.The cDNA sequence was 2169 bp in length,encoding 722 amino acids and had a high homology(80%-97%) with PAL genes from other plants.The deduced amino acid sequence of FePAL contained the typical sequence(GTTTASGDLVPLSYIA) in PAL activity center region,deamination residues and catalytic active sites.Phylogenetic tree analysis revealed that FePAL was most closely related to the PAL of Fagopyrum tataricum.

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Available abstract

The cDNA ORF sequence of phenylalanine ammonia-lyase gene(FePAL) from Fagopyrum esculentum was cloned by RT-PCR.The cDNA sequence was 2169 bp in length,encoding 722 amino acids and had a high homology(80%-97%) with PAL genes from other plants.The deduced amino acid sequence of FePAL contained the typical sequence(GTTTASGDLVPLSYIA) in PAL activity center region,deamination residues and catalytic active sites.Phylogenetic tree analysis revealed that FePAL was most closely related to the PAL of Fagopyrum tataricum.

Key concepts: Fagopyrum, Phenylalanine ammonia-lyase, Fagopyrum tataricum, Complementary DNA, Biology, Peptide sequence, Gene, Sequence analysis

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Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase(PAL) Gene from Fagopyrum esculentum — Research Paper | ScholarLens