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Cloning and sequence analysis of chalcone synthase gene Chs from Fagopyrum esculentum

Li Cheng

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Abstract

The cDNA ORF sequence of chalcone synthase gene(FeChs)was amplified by RT-PCR from Fagopyrum esculentum.Its accession number is GU172166.1 on GenBank.The anlyasis indicated the cDNA sequence was 1 179 bp,encoding 392 amino acids and had a homlogy 78%-92% with other plants'CHS genes.The deduced amino acids sequence of FeChs contained the highly conserved catalytic active sites,and the typical Tag sequence of CHS with GFGPG.

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What this paper is about

The cDNA ORF sequence of chalcone synthase gene(FeChs)was amplified by RT-PCR from Fagopyrum esculentum.Its accession number is GU172166.1 on GenBank.The anlyasis indicated the cDNA sequence was 1 179 bp,encoding 392 amino acids and had a homlogy 78%-92% with other plants'CHS genes.The deduced amino acids sequence of FeChs contained the highly conserved catalytic active sites,and the typical Tag sequence of CHS with GFGPG.

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Available abstract

The cDNA ORF sequence of chalcone synthase gene(FeChs)was amplified by RT-PCR from Fagopyrum esculentum.Its accession number is GU172166.1 on GenBank.The anlyasis indicated the cDNA sequence was 1 179 bp,encoding 392 amino acids and had a homlogy 78%-92% with other plants'CHS genes.The deduced amino acids sequence of FeChs contained the highly conserved catalytic active sites,and the typical Tag sequence of CHS with GFGPG.

Key concepts: Chalcone synthase, Fagopyrum, GenBank, Complementary DNA, Gene, Biology, Cloning (programming), Accession number (library science)

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Cloning and sequence analysis of chalcone synthase gene Chs from Fagopyrum esculentum — Research Paper | ScholarLens