Expression and Clinical Significance of Osteopontin in Pancreatic Carcinoma Using Tissue Microarray

Objective To investigate the expression of phosphorylated osteopontin (OPN) in pancreatic ductal adenocacinoma(PDAC) by high-throughput tissue microarray technique,as well as its relationship with the clinicopathologic features besides the prognostic significance of OPN expression in PDAC. Methods The pancreatic cancer tissue microarray was composed of 88 specimens of the pan-creatic adenocarcinoma and 11 normal pancreatic tissues as the control subjects. Two steps immunohis-tochemistry method was performed to examine expressions of OPN. In addition,53 of the 88 cases were followed-up from 2 months to 6 years,in which the survival was analyzed. Results Positive expression of OPN in tumors was showed 54(61.36%). The expression of OPN was significantly associated with late TNM stage and lymph node metastasis(P0.01),but there was no distinct difference found in tumor location,size and pathologic grade (P0.05). Univariate and multivariate analysis revealed that OPN is a prognostic indicator for PDAC. Conclusion The pathogenesis,development and metastasis of pancreat-ic cancer is associated with OPN. The expression of OPN is a significant prognostic indicator for PDAC.