Relationship between partial hepatectomy,apoptosis and liver reserve function in rats with the liver cirrhosis

Objective To study the relationship between hepatocellular apoptosis and liver reserve function after partial hepatectomy in the liver cirrhosis of rats.Methods 40 male Wistar rats were randomly divided into 4 groups,three groups were experimental groups,the lastest one is corresponding control group,developing models of liver cirrhosis rat of with complex factors(CCL4,alcohol),and the control group with saline.The rats of experimental groups were killed after 15 weeks respectively,So did the corresponding control group at the same time.The experimental groups were the partial hepatectomy groups.The blood was taken for the determination of common hepatic function.The livers were taken for pathologic examination and in situ TUNEL for detection of apoptosis. Results The expression of hepatocellular apoptosis of the partial hepatectomy were higher than preoperative group:15%(34.17±11.8)vs(25.94±7.24)、30%(44.57±15.49)vs(26.58±8.92)、45%(49.71±19.98)vs(27.16±5.94)(P0.05).Conclusion Hepatocellular apoptosis index and the volume of resected liver were positively correlated.To study hepatocellular apoptosis may be of great importance to predict liver reserve function.