Optimization of the Rooting Medium of Lonicera japonica Test-Tube Plantlet

[Objective]To screen the optimal rooting medium of Lonicera japonica test-tube plantlet. [Method]The axillary bud of Lonicera japonica was used as explant and the effects of different hormones( NAA,IBA,MET) on rooting were studied through the orthogonal test. [Result]The effects of different hormones on rooting radio was NAA IBA MET,the optimal medium of rooting radio was B5 + NAA 0. 05 mg / L+ IBA 0. 2 mg / L + MET 1. 0 mg / L. The effects of different hormones on the main root amount was IBA NAA MET,the optima medium of the main root amount was B5 + IBA 0. 4 mg / L + NAA 0. 2 mg / L + MET 0. 5 mg / L. The effects of different hormones on the fibrous root amount was NAA IBA MET,the optima medium of fibrous root amount was B5 + NAA 0. 05 mg / L + IBA 0. 4 mg / L + MET 0. 5 mg / L. [Conclusion]The optimal rooting medium for Lonicera japonica test-tube plantlet was obtained,which will provide a basis for the industrial seedling of Lonicera japonica.