Optimization of Rooting Medium of the Plantlets in Anthurium andraeanum
Niu Rui-h
Abstract
Niu Rui-h
Abstract
The research aimed to optimize the rooting technology system of the plantlets in Anthurium andraeaum. [Method]Plantlets inducted from Anthurium callus were used as explants,and the influence of auxin NAA and IBA,sucrose and activated charcoal were investigated through the method of orthogonal test L16(45). [Result]The results showed that the suitable medium for inducing roots was MS medium with IBA 0. 5 mg /L,activated charcoal 0. 7 g /L and sugar 30 g /L,and the induce effect of auxin IBA was better than that of NAA. Based on the Extremum analysis results,four factors all affected the rooting,and their effects on plantlet rooting was IBA active charcoal NAA sucrose,though didn't reach a significant difference levels. [Conclusion] The optimal combination for Anthurium plantlets rooting medium was A3B4C3D4,which is MS medium with 0.2 mg/L NAA,0.5 mg/L IBA,0.5 g/L activated charcoal and 30 g/L sugar.
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The research aimed to optimize the rooting technology system of the plantlets in Anthurium andraeaum. [Method]Plantlets inducted from Anthurium callus were used as explants,and the influence of auxin NAA and IBA,sucrose and activated charcoal were investigated through the method of orthogonal test L16(45). [Result]The results showed that the suitable medium for inducing roots was MS medium with IBA 0. 5 mg /L,activated charcoal 0. 7 g /L and sugar 30 g /L,and the induce effect of auxin IBA was better than that of NAA. Based on the Extremum analysis results,four factors all affected the rooting,and their effects on plantlet rooting was IBA active charcoal NAA sucrose,though didn't reach a significant difference levels. [Conclusion] The optimal combination for Anthurium plantlets rooting medium was A3B4C3D4,which is MS medium with 0.2 mg/L NAA,0.5 mg/L IBA,0.5 g/L activated charcoal and 30 g/L sugar.
Key concepts: Anthurium, Plantlet, Sucrose, Activated charcoal, Auxin, Explant culture, Callus, Charcoal