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2013•Medical Science Journal of Central South ChinaRequires access

Proliferation Inhibition and c-myc Expression Down-Regulated by Diallyl Disulfide in HL-60 Cells

Zhao Zhan-zhi

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Abstract

Objective To investigate the effects of DADS on proliferation inhibition and on c-myc protein expression in human myeloid leukemia cell line HL-60 cells.Methods HL-60 cells were exposed to DADS at different dosages.Growth inhibition was detected by MTT assay and cell growth.The expression of c-myc was measured by Western blotting.Results After treatment with DADS,the growth of HL-60 cells were suppressed in a concentration-dependent and time-dependent manner(P0.05).Incubation of HL-60 cells with DADS(20 μmol/L)for 12 h could decrease the expression of c-myc.Conclusion DADS could inhibit HL-60 cells proliferation,its mechanism may be closely related with adjusting expression level of the c-myc.

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What this paper is about

Objective To investigate the effects of DADS on proliferation inhibition and on c-myc protein expression in human myeloid leukemia cell line HL-60 cells.Methods HL-60 cells were exposed to DADS at different dosages.Growth inhibition was detected by MTT assay and cell growth.The expression of c-myc was measured by Western blotting.Results After treatment with DADS,the growth of HL-60 cells were suppressed in a concentration-dependent and time-dependent manner(P0.05).Incubation of HL-60 cells with DADS(20 μmol/L)for 12 h could decrease the expression of c-myc.Conclusion DADS could inhibit HL-60 cells proliferation,its mechanism may be closely related with adjusting expression level of the c-myc.

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Available abstract

Objective To investigate the effects of DADS on proliferation inhibition and on c-myc protein expression in human myeloid leukemia cell line HL-60 cells.Methods HL-60 cells were exposed to DADS at different dosages.Growth inhibition was detected by MTT assay and cell growth.The expression of c-myc was measured by Western blotting.Results After treatment with DADS,the growth of HL-60 cells were suppressed in a concentration-dependent and time-dependent manner(P0.05).Incubation of HL-60 cells with DADS(20 μmol/L)for 12 h could decrease the expression of c-myc.Conclusion DADS could inhibit HL-60 cells proliferation,its mechanism may be closely related with adjusting expression level of the c-myc.

Key concepts: Diallyl disulfide, Cell growth, Molecular biology, Cell culture, MTT assay, Growth inhibition, Myeloid leukemia, Blot

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