Determination of quercetin and kaempferol in different medicinal parts of Acanthopanax henryi by RP-HPLC

Objective To establish a RP-HPLC method for the determination of quercetin and kaempferol in different medicinal parts of Acanthopanax henryi.Methods The analysis was performed on an AT.Lichrom ODS-C18 column (250 mm×4.6 mm,5μm) with methanol-phosphoric acid-water (50:0.2:49.8) as mobile phase.The flow rate was 1.0 mL/min.The column temperature was 30℃,and the detection wavelength was 360 nm.Results The established method showed good linearity for quercetin and kaempferol over the range of 0.018—0.720 μg (r=0.999 6) and 0.016—0.640 μg (r=0.999 7),respectively.The average recovery of the method for quercetin and kaempferol were 98.41% with the RSD 0.91% and 98.06% with the RSD 1.39%,respectively.The highest content of quercetin and kaempferol was in the leaves of A.henryi,followed by stems,and undetected in roots.Conclusion Contents of quercetin and kaempferol in different medicinal parts of A.henryi are determined.The established HPLC method shows good stability and high accuracy,and can be used for the quantitative analysis of quercetin and kaempferol in A.henryi.