Research on the Induction of Callus and Adventitious Buds in the Anther Culture of Tomato

In order to establish a stable and high efficient embryogenic callus induction system in tomato,via anther culture in vitro and design various treatment,six tomato varieties were used in the experiment to study the effects of plant growth regulators,sucrose,silver nitrate level,genotypes and medium on induction of anther callus and the formation of adventitious bud.The results show that the optimized hormone combination of callus inducement was 1.0mg/L 2,4-D and 0.5mg/L KT;30g/L sucrose could enhance the callus inducement about 20.24% on Liger87-5;10μmol/L silver nitrate could reduce browning of the tissues about 69.23%,When the concentration of silver nitrate exceeded 50μmol/L,the browning of the tissues would increased and the callus inducement would decreased imperceptibility;the callus was related to the genotypes,in the same culture condition,the induction rate of Liger87-5 was as high as 21.43%,the induction rate of FB2 was only 0.46%;The callus inducement frequency with addition of 175g/L potato milk and 0.1% active carbon decreased 35.14%、95.48% in the six different materials respectively.Medium of 6-BA 2mg/L+ZT 0.5mg/L+IAA 0.1mg/L could make callus dedifferentiate into adventitious bud,and the rate of callus inducement was 15.15%.