Determination of nitrofuran metabolites in abalone by high performance Liquid Chromatography-Tandem Mass Spectrometry

A method has been developed for four metabolites of nitrofuran antibiotics such as 3-amino-2-oxazolidinone(AOZ),5-morpholino-methyl-3-anmino-2-oxazolidinone(AMOZ),semicarbazid(SEM) and 1-aminohydantoin(AHD) by high performance Liquid Chromatography-Tandem Mass Spectrometry.Abalone tissues samples were hydrolyzed with 0.2mol/L HCl,and derivatived at 37℃ for sixteen hours with 2-nitrobenzaldenhyde.The derivatived solutions were adjusted to pH 7.0~7.5,and extracted with ethyl acetate.Analyte identification and quantification were performed using selected reaction monitoring(SRM) with one precursor ion and two product ions as identifiers and electrospray ionization in positive mode.There are good linear correlations between the peak areas and concentrations of the four metabolites of nitrofuran antibiotics(the calibration coefficients are above 0.99),the dynamic linear range is 0.5~20μg/kg.The limit of detection is 0.5μg/kg for AOZ,AMOZ,SEM,AHD.The average recoveries ranged from 75.2% to 102.1% and their relative standard deviations are between 3.56 % and 12.6 % The method can be used to identify and quantify the metabolites of nitrofuran antibiotics in aquatic products with satisfactory sensitivity,accurcy and precision.