The establishment of stable expression of GRIM-19 in Hela cell line and its inhibitive effect on STAT3 expression

Objective:To establish the stable Hela cell lines with a persistent expression of GRIM-19,explore the expression of GRIM-19 and it's effect on the downstream gene-signal transducer and activator of transcription 3(STAT3).Methods:The recombinant plasmid was transfected into Hela cells by liposome method,then the positive transfected cells were screened by G418.The expression of GRIM-19 and STAT3 in Hela/GRIM-19 cells in transfected Hela cells was identified by RT-PCR and Western blot.Results:The transfencted Hela cell line was established suscessfully,which can express GRIM-19 highly.The high expression of GRIM-19 in Hela cells can obviously downregulate the expression of STAT3.Conclusion:The high expression of GRIM-19 in Hela cells can inhibit the expression of STAT3.