Study of the inhibitory growth and reversing effect DDP resistance of medroxyprogesterone acetate on the ovarian cancer cell line CoC1/cDDP

Objective:To study the effect of medroxyprogesterone acetate (MPA) on the inhibitory growth and reversing DDP resistance in drug resistant epithelial ovarian carcinoma cell line in vitro.Methods:CoC1/cDDP cell,treated by different concentrations of MPA in different time course, were examined for the growing inhibiting rates with methyl thiazolyl tetrazolium(MTT);treated by MPA?DDP and MPA+DDP were estimated for the growing inhibiting rates by MTT;apoptosis percentage and cell cycle phase distribution were studied by flow cytometry(FCM).Results:CoC1/cDDP cell growing inhibiting rates by MPA were significantly different in dose-dependent and time-dependent manners(P0.01).The inhibition effect of MPA combined with DDP was more obvious than either one used separately;the number of cells in G0/G1?G2/M phase was elevated,the number of cells in S phase was obviously decreased(P0.01),the apoptotic rate rose after treatment by MPA combined with DDP?Conclusion:MPA can inhibit the growth of CoC1/cDDP cells and can reverse the tolerance of CoC1/cDDP cell lines to cisplatin.