Application of atrophic gastritis diagnosis by detecting serum pepsinogen and gastrin

Objective To establish optimal atrophic gastritis diagnoses threshold of serum pepsinogen and gastrin-17 in our own laboratory,investigate the relationship with nonatrophic gastritis and H. pylori-IgG.Methods The concentration of serum PG and G-17 were detected by enzyme-linked immunosorbent assay( ELISA) in 38 patients with atrophic gastritis,40 patients with nonatrophic gastritis and 35 healthy subjects as control,HP-IgG antibody were detected by method of colloidal gold,the optimal cutoff-value of screening atrophic gastritis markers were obtained by receiver operating characteristic( ROC) curves. Results The serum levels of PG-Ⅰ,PGR and G-17 were significant decreased in atrophic gastritis group,only serum PG-Ⅰ decreased in nonatrophic gastritis group,the optimal diagnostic cutoff-value of PG-Ⅰ,PGR and G-17 were 74μg /L( sensitivity0. 632,specificity 0. 857),6. 2( sensitivity 0. 658,specificity 0. 943),5. 1pmol /L( sensitivity 0. 816,specificity0. 676) respectively,the sensitivity of PG-Ⅰ 74μg /L was up to 0. 803 when PG-Ⅱ 9. 6μg /L,and the specificity was down to 0. 826. The serum levels of PG-Ⅰand G-17 with HP positive were remarkable higher and then serum PGR level was lower than that with HP negative in healthy control group,there were no difference with HP infection in atrophic gastritis and nonatrophic gastritis groups. Conclusions We provide the optimal diagnostic cutoff-value of PG-Ⅰ,PGR and G-17 for screening and auxiliary diagnosis of atrophic gastritis and compared all the markers with nonatrophic gastritis,HP infection would influence the levels of pepsinogen and gastrin in healthy control group.