THE EXPRESSION OF p53 and HPV - 16,18 E6 PROTEINS AND ITS SIGNIFICNACE IN CERVICAL CARCINOMA

Objective To study the expression of p53 and HPV 16, 18 E6 proteins and its significance in cervical carcinoma.Methods Immuno histochemical SP method was used to examine the expression of p53 and HPV 16,18 E6 proteins in 68 cases of cervical carcinoma and 8 cases of epidermal tissues of chronic cervistis.Results The positive expression of p53 and HPV 16, 18 E6 proteins were observed in 17 and 60 in the 68 cervical carcinoma with positive rates of 25.0% and 88.2% respectively. Expression of p53 was associated with histological types:the positive rates were 83.3% in adenocarcinoma and 17.2% in squamous cell carcinoma ( P 0 01). It was also related to clinical stages and was higher as clinical stage increased ( P 0 01), but is was not associated with tumor grades and lymphnodes metastasis ( P 0 05). Positive expression of HPV 16,18 E6 proteins were correlated neither with tumor grades and lymphnodes metastasis nor with histological type and clinic stages ( P 0 05). None of chronic cervistits epitehelium showed positive stain of p53 and HPV 16,18 E6 proteins. Conclusion Positive expression of p53 was increased in cervical adenocarcinoma and it was higher as the clicical stage increases. This may be the result of p53 gene mutation, or other cellular protein binding to p53 protein may protect against HPV 16, 18 E6 induced p53 protein degradation, and thus p53 protein level may be elevated through an increase in its stability.$$$$