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Analysis of Immunophenotype in Acute Myeloid Leukemia by Multiparameter Flow Cytometry

Pei Yan

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Abstract

To evaluate the immunophenotype of acute leukemia patients, the surface and cyto-plasmic antigen expression in 162 cases of acute leukemia were analyzed by multiparameter flow cytometry and CD45/SSC gating. The results showed that CD117 (94. 9%),CD13 (88. 5%) and CD33(70. 5%) were mainly expressed in ANLL patients; cCD79a(100%) ,CD19(92. 1%) were chiefly expressed in B-ALL patients, and in T-ALL patients, cCD3(100%) and CD2(83. 3%) were expressed; For the expression of lymphoid differentiation antigen Ly+ANLL, CD7 (56. 2%) and CD19(31. 2%) were chiefly found, and for myeloid antigen My+ALL, CD13(88. 9%) and CD33(27. 8%) were detected. In conclusion, multiparameter flow cytometry and three-color direct immunofluorescence staining methods may be of important clinical significance in diagnosis, therapy and prognosis of acute leukemia.

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What this paper is about

To evaluate the immunophenotype of acute leukemia patients, the surface and cyto-plasmic antigen expression in 162 cases of acute leukemia were analyzed by multiparameter flow cytometry and CD45/SSC gating. The results showed that CD117 (94. 9%),CD13 (88. 5%) and CD33(70. 5%) were mainly expressed in ANLL patients; cCD79a(100%) ,CD19(92. 1%) were chiefly expressed in B-ALL patients, and in T-ALL patients, cCD3(100%) and CD2(83. 3%) were expressed; For the expression of lymphoid differentiation antigen Ly+ANLL, CD7 (56. 2%) and CD19(31. 2%) were chiefly found, and for myeloid antigen My+ALL, CD13(88. 9%) and CD33(27. 8%) were detected. In conclusion, multiparameter flow cytometry and three-color direct immunofluorescence staining methods may be of important clinical significance in diagnosis, therapy and prognosis of acute leukemia.

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Available abstract

To evaluate the immunophenotype of acute leukemia patients, the surface and cyto-plasmic antigen expression in 162 cases of acute leukemia were analyzed by multiparameter flow cytometry and CD45/SSC gating. The results showed that CD117 (94. 9%),CD13 (88. 5%) and CD33(70. 5%) were mainly expressed in ANLL patients; cCD79a(100%) ,CD19(92. 1%) were chiefly expressed in B-ALL patients, and in T-ALL patients, cCD3(100%) and CD2(83. 3%) were expressed; For the expression of lymphoid differentiation antigen Ly+ANLL, CD7 (56. 2%) and CD19(31. 2%) were chiefly found, and for myeloid antigen My+ALL, CD13(88. 9%) and CD33(27. 8%) were detected. In conclusion, multiparameter flow cytometry and three-color direct immunofluorescence staining methods may be of important clinical significance in diagnosis, therapy and prognosis of acute leukemia.

Key concepts: Immunophenotyping, Medicine, CD117, CD33, Flow cytometry, Myeloid leukemia, Acute leukemia, Leukemia

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Analysis of Immunophenotype in Acute Myeloid Leukemia by Multiparameter Flow Cytometry — Research Paper | ScholarLens