Expression and significance of human cervical cancer oncogene(HCCR) mRNA in hepatocellular carcinoma tissues

[Objective] To observe the human cervical cancer oncogene(HCCR) mRNA levels in hepatocellular carcinoma(HCC) and para-cancerous tissues,and peripheral blood monouclear cells(PBMC) of HCC patients and normal controls.[Methods] The cancerous and para-cencerous tissues from HCC patients and the PBMC from HCC patients and normal controls were collected.Total RNA was extracted by using Trizol reagent.Reverse-transcription polymerase chain reactions(RT-PCR) were performed with the HCCR and beta-actin primers to detect the different HCCR mRNA levels in these samples by semi-quantitative analysis.[Results] The relative expression levels of HCCR mRNA were higher in the cancerous tissues than those in the para-cancerous tissues(0.776±0.101 and 0.470±0.154,P0.05).In PBMC,HCCR mRNAs were detected in the HCC group,but were not detected in the normal control groups.The relative expression levels of HCCR mRNA of the PBMC in HCC group(0.256±0.069) were higher than that in the normal ones(P0.05).[Conclusion] HCCR mRNAs show higher expression levels in the group of HCC patients,indicating the HCCR gene maybe participates in genesis and development of HCC,and suggesting the HCCR gene has some correlation with the pathological progress of HCC progress.