2010•Traditional Chinese Drug Research and Clinical PharmacologyRequires access

Determination of Luteolin in Zhuangyao Qingre Granula by HPLC

Peng Xin

Abstract

Objective To establish the determination method for luteolin in Zhuangyao Qingre Granula.Methods The content of luteolin was determined by HPLC,the analytical column was symmetry C18(4.6 mm×150mm,5μm),the mobile phase consisted of methanol-0.2%phosphatic acid(50∶50)at a flow rate of 1.0 mL·min-1,the detection wavelength was 350 nm,and the column temperature was at room temperature.Results Luteolin injection volume in the range of 0.16μg to 0.80μg showed good linear relationship(r=0.999 7),the average recovery rate was 97.6%(RSD=1.75%).This method met the analysis requirement.Conclusion The established HPLC method is reproducible and effective,and is suitable for the determination of luteolin in Zhuangyao Qingre Granula.

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Available abstract

Key concepts: Luteolin, Chromatography, Chemistry, High-performance liquid chromatography, Content determination, Recovery rate, Flavonoid, Biochemistry