Simultaneous Determination of the Content of Flavonoid Compound in Two Different Formulation of Ginkgo Leaf Extract by HPLC

Objective To determine the content of three flavonoid compound(quercetinic acid,kaempferol and isorhamnetin) in two different formulation of ginkgo leaf extract.Methods HPLC method was used to determine the content of quercetinic acid,kaempferol and isorhamnetin.Results The linear correlation of the peak area and the concentration was observed for quercetinic acid(6.4~32.0μg/mL),kaempferol(4.4~22.0μg/mL) and isorhamnetin(3.2~16.0μg/mL) respectively.The recovery was 98.9%~100.4% with RSD1.29%.Conclusion The present experiment established a HPLC method for simultaneously determining three ingredients of quercetinic acid,kaempferol and isorhamnetin.The method is convenient,accurate and suitable for the quality control of the tablets and injections of ginkgo leaf extract.