Determination of Total Flavonoid Glycoside in Compound GinKgo Leaf Capsule by HPLC

OBJECTIVE:To establish an HPLC method for the determination of total flavonoid glycoside in Compound ginkgo leaf capsule.METHODS:The quantitative analysis of total flavonoid glycoside was performed on Shiseido MG C18(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanol-0.4% phosphoric acid solution(68∶32).The detective wavelength was 378 nm.And the quantitation was carried out using external standard method.RESULTS:The linear ranges of Quercetin,Kaempferol,and Isorhamnetin were 6～120 μg·mL-1(r=0.999 97),3～60 μg·mL-1(r=0.999 98),and 1～20 μg·mL-1(r=0.999 96),respectively.The contents of total flavonoid glycoside in three batches of samples were 10.14,10.01,and 10.07 mg,respectively.CONCLUSION:The method is rapid,accurate and reproducible,and suitable fore the quality control of Compound ginkgo leaf capsule.