Pathogenesis of kidney injury in severe acute pancreatitis rats

Objective To explore the relationship between the changes of plasma cytokines,LPS,angiotensin Ⅱ and the renal injury in severe acute pancreatitis(SAP) rats.Methods SD rats were divided randomly into sham operation group(n=30) and SAP group(n=30).SAP animal model was established in adult male rats by retrograde injection of 4% sodium laurocholate l ml/kg in biliopancreatic duct.Morphology of pancreas and kidneys were observed.Plasma levels of TNF,IL-6,LPS,AT Ⅱ and BUN,Cr were measured at various time points.Results The plasma levels of TNF,IL-6,LPS and AT Ⅱ was increased after SAP induction and there was a significant increase after 24 h,their levels reached a peak at 48 h,and remained at high level at 72 h.Conclusion The levels of TNF、IL-6、LPS、AT Ⅱ was increased and the renal function was injured in the early stage of SAP.Cytokines,LPS,and activated renin-angiotensin system might be involved in pathogenesis of kidney injury following SAP.The renal function damage was increased with severity of SAP.There was a close relationship between kidney injury and pancreatitis.