Protective effects of interleukin-lO on renal function in rats with severe acute pancreatitis

Objective To investigate the protective effects of interleukin-10(IL-10)on renal function in rats after severe acute pancreatitis(SAP).Methods A total of 54 male SD rats were ran- domized into the sham-operated group(SO),SAP group,SAP plus IL-10 treatment group(SAP+IL- 10).SAP model was established by injecting 5% sodium taurodeoxyeholate into biliary-pancreatic duct.Serum amylase,tumor necrosis factor-alpha(TNF-α),IL-6,urea nitrogen(BUN),creatinine (Cr),volume of ascites and histological changes of pancreas and kidney were determined at 12,24h after induction of SAP.Intercellular adhesion molecule-1(ICAM-1)mRNA in kidney was analyzed by RT-PCR.Results The levels of serum TNF-αand IL-6 in SAP group increased significantly after SAP onset.The expression of ICAM-1 mRNA in kidney was up-regulated(P0.01).BUN and Cr elevated persistently at 12,24h after induction of SAP.As compared with SAP group,the levels of serum TNF-α,IL-6,BUN and Cr remarkably decreased,the expression of ICAM-1 mRNA down-reg- ulated,and the pathological changes in kidney attenuated in SAP+IL-10 group.Conclusion IL-10 can inhibit serum levels of TNF-αand IL-6,down-regulate ICAM-1 gene expression and ease SAP re- nal function disorder.