2010Food Science and Technology InternationalRequires access

Extraction of cellulase by aqueous two-phase system

Zhiwei Yang

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Abstract

The effects of concentration of polyethylene glycol,ammonium sulfate and pH on extraction of cellulase from industrial cellulase in aqueous two-phase system of PEG-4000 and ammonium sulfate were studied.The results show that: The conditions of aqueous two-phase systems extraction are as folows: concentration of(NH4)2SO4 is 8%,concentration of PEG4000 26%,pH4.8,The partition coefficient K and the recovery rate were 5.21 and 79.4% respectively.

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The effects of concentration of polyethylene glycol,ammonium sulfate and pH on extraction of cellulase from industrial cellulase in aqueous two-phase system of PEG-4000 and ammonium sulfate were studied.The results show that: The conditions of aqueous two-phase systems extraction are as folows: concentration of(NH4)2SO4 is 8%,concentration of PEG4000 26%,pH4.8,The partition coefficient K and the recovery rate were 5.21 and 79.4% respectively.

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Available abstract

The effects of concentration of polyethylene glycol,ammonium sulfate and pH on extraction of cellulase from industrial cellulase in aqueous two-phase system of PEG-4000 and ammonium sulfate were studied.The results show that: The conditions of aqueous two-phase systems extraction are as folows: concentration of(NH4)2SO4 is 8%,concentration of PEG4000 26%,pH4.8,The partition coefficient K and the recovery rate were 5.21 and 79.4% respectively.

Key concepts: Cellulase, Ammonium sulfate, Extraction (chemistry), Aqueous solution, Polyethylene glycol, Chemistry, Partition coefficient, Aqueous two-phase system

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